The concept and architecture of Internet of Things (IoT) is getting progresses every day. This is proposed to various objects globally which is accessible from the internet and gets integrated to many different portable devices. This Paper presents the four-layer architecture of communication protocols in which it is oriented to medical applications. Different portable devices (Sensors) of medical application have been used for measuring vital signs (Temperature, Blood Pressure, Respiration rate, Pulse rate and Electrocardiogram, etc.,) of human body, these sensors will give series of information or data about the vital signs measured. This Measured data (information) is transmitted to Android based mobile application (Mobile App) through which the data can be monitored via Bluetooth (IEEE 802.15.1) at 2.4 GHz and the device is controlled by the single board computers. The Middleware services for maintaining (Collecting and retrieving) of databases in the server are studied.