3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
77
LEMNA MINOR INFLUENCE IN THE TREATMENT OF ORGANIC
POLLUTION OF THE INDUSTRIAL EFFLUENTS
Karina Alvarado
Universidad Tecnológica del Perú, Lima, (Perú).
E-mail: c10208@utp.edu.pe ORCID: https://orcid.org/0000-0001-7142-4212
Doris Esenarro
National University Federico Villarreal, Lima, (Perú).
E-mail: desenarro@unfv.edu.pe ORCID: https://orcid.org/0000-0002-7186-9614
Ciro Rodriguez
National University Mayor de San Marcos, Lima, (Perú).
E-mail: crodriguezro@unmsm.edu.pe ORCID: https://orcid.org/0000-0003-2112-1349
Wilson Vasquez
Universidad Tecnológica del Perú, Lima, (Perú).
E-mail: c18347@utp.edu.pe ORCID: https://orcid.org/0000-0001-7064-028X
Recepción:
08/06/2020
Aceptación:
20/07/2020
Publicación:
14/09/2020
Citación sugerida:
Alvarado, K., Esenarro, D., Rodriguez, C., y Vasquez, W. (2020). Lemna minor inuence in the treatment of organic
pollution of the industrial euents. 3C Tecnología. Glosas de innovación aplicadas a la pyme, 9(3), 77-97. https://doi.
org/10.17993/3ctecno/2020.v9n3e35.77-97
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
78
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
ABSTRACT
The purpose of the research was to determine the inuence of industrial wastewater treatment using
the Lemna Minor aquatic plant. Certain varieties of macrophyte plants can absorb or retain various
contaminants. Thanks to this, it has been determined that the variety known as Lemna Minor presents
this type of property. Three treatment trials were carried out varying the amounts of Lemna Minor (100,
200, and 300g). They are keeping constant the retention time of 10 days that were analyzed at 3, 6, and
10 days after the treatment and with a constant volume of the residual euent. The results indicate that
in terms of the parameters that determine organic contamination, BOD was reduced by (61 %); COD
was reduced by (68 %) and the concentration of total suspended solids by (61 %).
KEYWORDS
Lemna minor, Organic pollution, Industrial euents, Wastewater treatment, Macrophyte plants.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
79
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
1. INTRODUCTION
The euents from industrial processes coming from dyestus present organic matter expressed as
chemical oxygen demand (COD), biochemical oxygen demand (BOD), and total suspended solids (TSS).
The bathing ratios (BR) in these types of processes comprise 1:7 to 1:12; this leads to the use of large
quantities of water. These euents must be treated before being introduced into the industrial drainage
system, to comply with national regulations related to the use of public sewage systems and to avoid
contamination of the receptors.
The main benet of treatment systems with aquatic plants is their low cost of construction and
maintenance, as well as their simplicity of operation using an available resource.The presence of a
large amount of organic contamination depletes the oxygen in the water, resulting in a decrease in the
appropriate conditions for life, producing fermentations that lead to bad odors. The sedimented solids,
many of them are toxic because they carry heavy metals such as Cu, Mn, Cd and Cr (Hoyos et al., 2016).
In this research thesis: Inuence of the use of Lemna minor in the treatment of organic contamination
of industrial euents in Cotexsur. The objective of the present work was to determine the inuence
of the treatment of industrial wastewater from the company Cotexsur, using the aquatic plant Lemna
Minor. The research was of an applied type and experimental design with a quantitative approach. The
sample was taken considering the convenience and criteria previously evaluated by the types of analysis
performed and the treatment proposed (Sun et al., 2020; Walsh et al., 2020).
The theoretical bases that support the research, thus, the advantages of using the Lemna Minor, previous
denitions of textile dyes, and parameters for measuring organic contamination. The research was also
carried out with Lemna Minor, which has a percentage of removal expressed as COD 72.57 %, BOD5
73.36 %, total solids 75.21 % and in the rst six days of treatment with dierent masses of 100g, 200g of
Lemna Minor there is a high percentage of decrease in the concentrations of the mentioned parameters
(Hoyos et al., 2016; Li et al., 2020).
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
80
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
2. METHOD AND INSTRUMENTS
The measurements obtained are analyzed (often using statistical methods).The sample was considered
according to convenience. The criteria previously evaluated, the types of analysis performed, and the
treatment proposed, therefore the sample was non-probabilistic.
The total volume of the sample was 70 liters from the industrial textile euents. These 70 liters will be
divided into 3 types of treatment: 100g, 200g, and 300g of Lemna Minor and each of the treatments
with 7 experimental runs (Coronel, 2016).
For the collection of data, as Rodriguez et al. (2020), which made it possible to have better control over
the data that allowed the characterization of the euents from Cotexsur. The following equipment was
used to obtain the concentrations of the parameters:
HI 2210 potentiometer.
COD digester DRB 200.
Colorimeter DR 900.
Equipment for determining BOD.
Analytical balance NBL 124 E.
Digital sterilization and drying oven DHG 9023 A.
Button lid dryer, 150 mm.
The equipment and materials for the analyses were provided by the laboratory of the Universidad
Autónoma del Perú, which allowed to determine: PH, Biochemical Oxygen Demand, Chemical Oxygen
Demand, and Total Suspended Solids (Jojoa, Rodríguez, & Cardona, 2015).
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
81
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
2.1. 1ST COLLECTION AND IDENTIFICATION OF LEMNA MINOR
From the lagoons adjacent to the villa marshes in the Chorrillos district, samples of Lemna Minor were
collected to be used in the experimental runs, considering the following conditions:
Have good pigmentation
That they do not present an anomaly in any of their parts.
The laboratory where the analyses were carried out has adequate ventilation and sunlight. The
temperature and humidity in the experimental runs were:
Maximum temperature: 210C
Minimum temperature: 180C
Humidity: 89%.
2.2. 2ND COLLECTION AND ANALYSIS OF SAMPLES OF THE INDUSTRIAL EFFLUENTS
OF COTEXSUR
Wastewater analysis was performed according to standardized methods (Standard Methods for
the examination wáter and wastewater, APHA) and instruments, equipment, reagents, which are
recommended in the methods. Table 1 details the method or technique applied for each analysis
parameter.
Table 1. Health quality monitoring protocol, according to DIGESA (Dirección General de Salud Ambiental).
Parameters /Dimension of the
dependent variable
Method / Technique Bottle material
Biochemical Oxygen Demand Dilution Plastic or glass
Biochemical Oxygen Demand Colorimetric Plastic or glass
Total suspended solids Gravimetric Plastic or glass
PH Electrometric Field Determination
Source: (Dirección General de Salud Ambiental e Inocuidad Alimentaria (DIGESA), s.f.).
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
82
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
4. RESULTS
4.1. DESCRIPTIVE
The results obtained in the development of the research are presented below:
Table 2. Distribution of average BOD concentrations vs. treatment time for 100 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (Days)
Media DBO
(ppm)
100 0 823
100 3 518
100 6 389
100 10 319
Source: authors’ own elaboration.
Table 2 and Figure 1 show the decrease in the average concentration of BOD as a function of time, for
a mass in contact of 100 g of Lemna Minor, the average BOD concentration in the initial time was 823
ppm; when in contact for 3 days the average concentration of BOD decreases to 518 ppm; for 6 days of
treatment the BOD concentration was 369 ppm, and for 10 days the BOD concentration was reduced
to a concentration of 319 ppm. (Esenarro et al., 2020).
Table 3. Distribution of average BOD concentration vs. treatment time for 200 g Mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (days)
Media DBO
(ppm)
200 0 823
200 3 468
200 6 346
200 10 389
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
83
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
In Table 3, we can see the decrease in the average concentration of the biochemical oxygen demand
as a function of time. For a mass in contact of 200 g of Lemna Minor, the average concentration for
the initial contact time was 823 ppm; when in contact for 3 days, the average concentration of the
biochemical oxygen demand decreased to 468 ppm; for 6 days of treatment, the BOD concentration
decrease to 346 ppm for 10 days, the BOD concentration was reduced to 389 ppm.
Table 4. Distribution of average BOD concentration vs. treatment time for 300 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (days)
Media DBO
(ppm)
300 0 823
300 3 429
300 6 331
300 10 533
Source: authors’ own elaboration.
In Table 4, the decrease in time-averaged BOD concentrations for a contact mass of 300 g of Lemna
Minor is shown. The average concentration for the initial treatment time was 823 ppm. This, when in
contact for 3 days, the average concentration of BOD decreases to 429 ppm. For 6 days of contact, the
BOD concentration was 331 ppm, and for 10 days, the BOD concentration was 533 ppm.
Table 5. Distribution of average BOD concentration vs. treatment time and mass of Lemna Minor.
Mass (g) Time (days)
BOD average
(ppm)
Standard
deviation
N
100
0 days
3 days
6 days
10 days
Total
833,57
517,71
389,43
318,57
512,07
26,018
34,028
29,205
20,354
198,242
7
7
7
7
28
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
84
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Mass (g) Time (days)
BOD average
(ppm)
Standard
deviation
N
200
0 days
3 days
6 days
10 days
Total
822,57
468,29
345,57
388,57
506,25
26,018
34,898
33,125
30,237
193,563
7
7
7
7
28
300
0 days
3 days
6 days
10 days
Total
822,57
429,43
331,43
532,86
529.07
26,018
27,367
15,736
17,995
188,367
7
7
7
7
28
Total
0 days
3 days
6 days
10 days
Total
822,57
471,81
355,48
413,33
515,8
24,683
48,041
36,049
94,092
191,336
21
21
21
21
84
Source: authors’ own elaboration.
Table 5 shows the averages of BOD concentration versus treatment time at dierent masses of Lemna
Minor. The results obtained according to ANOVA show that the greatest decrease in BOD concentration
was for a mass of 100 g of Lemna Minor and 10 days of treatment with a decrease in the concentration
of biochemical oxygen demand from 823 ppm to 319 ppm.
Figure 1. Average BOD concentration levels vs. treatment time and mass of Lemna Minor. Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
85
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Figure 1 shows the decrease in average BOD concentration for a treatment time of 10 days and mass
of 100 Lemna Minor g from 823 to 319 ppm. With a treatment of 200 g of Lemna Minor, the BOD
concentration decreases in 6 days from 823 ppm to 346 ppm and then increases in 10 days to 389 ppm,
the same behavior is obtained for treatment with 300 g of Lemna Minor. The BOD concentration
decreases in 6 days from 823 ppm to 331 ppm, concluding: a better treatment of organic contamination
expressed as BOD is obtained with a mass of 100 g of Lemna Minor.
Table 6. Distribution of average COD concentrations vs. treatment time for a mass of 100 g of Lemna Minor.
Mass Lemna
Minor (g)
Time (days)
DQO average
(ppm)
100 0 1747
100 3 1062
100 6 749
100 10 554
Source: authors’ own elaboration.
In Table 6, we can see the decrease in the average concentration of chemical oxygen demand as a
function of time for a treatment with a mass of Lemna Minor of 100 g of Lemna Minor, the average
concentration in the initial time was 1747 ppm; when being in contact for a period of 3 days, the average
concentration of chemical oxygen demand decreases to 1062 ppm; for 6 days of contact the COD
concentration was 749 ppm, and in 10 days a COD concentration of 554 ppm is obtained, obtaining a
considerable reduction.
Table 7. Distribution of average COD concentrations vs. treatment time for 200 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (days)
DQO average
(ppm)
200 0 1747
200 3 913
200 6 603
200 10 684
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
86
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Table 7 show the decrease in the time-averaged concentrations of the chemical oxygen demand for a
treatment with a Lemna Minor mass of 200 g Lemna Minor. The average concentration in the initial
time was 1747 ppm; when in contact with Lemna Minor for 3 days the average concentration of the
chemical oxygen demand decreases to 913 ppm; for 6 days of contact with Lemna Minor the COD
concentration was 603 ppm, and for 10 days an increase in the COD concentration to 684 ppm is
obtained.
Table 8. Distribution of average COD concentrations vs. treatment time for 300 g mass of Lemna Minor.
Mass Lemna Minor
(g)
Time (days)
DQO average
(ppm)
300 0 1747
300 3 1046
300 6 839
300 10 1250
Source: authors’ own elaboration.
Table 8 show the decrease in the time-averaged concentrations of chemical oxygen demand for a contact
mass of 300 g of Lemna Minor. The average concentration in the initial time was 1747 ppm; when in
contact with Lemna Minor for a period of 3 days the average concentration of the chemical oxygen
demand decreases to 1046 ppm; for 6 days of contact the COD concentration was 839 ppm, and for 10
days an increase in the COD concentration to 1250 ppm is obtained.
Table 9. Distribution of average COD concentrations vs. treatment time and mass of Lemna Minor.
MASA (gramos) TIEMPO (días) DQO average (ppm) Desviación estándar N
100 gramos
0 días 1747,14 85,968 7
3 dias 1061,71 59,905 7
6 dias 748,57 86,877 7
10 dias 554,29 76,345 7
Total 1027,93 467,129 28
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
87
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
MASA (gramos) TIEMPO (días) DQO average (ppm) Desviación estándar N
200 gramos
0 días 1747,14 85,968 7
3 dias 912,86 59,362 7
6 dias 602,86 51,223 7
10 dias 684,29 61,606 7
Total 986,79 465,936 28
300 gramos
0 días 1747,14 85,968 7
3 dias 1045,71 58,838 7
6 dias 838,57 49,473 7
10 dias 1250 96,609 7
Total 1220,36 350,571 28
Total
0 días 1747,14 81,556 21
3 dias 1006,76 88,581 21
6 dias 730 117,004 21
10 dias 829,52 318,535 21
Total 1078,36 438,263 84
Source: authors’ own elaboration.
In Table 9 the distribution of average COD concentrations vs. treatment time at dierent masses of
Lemna Minor is shown. The results obtained according to ANOVA were determined that the best
reduction of COD concentration was with a mass of 100 g of Lemna Minor and 10 days of treatment;
the chemical oxygen demand was reduced from 1747 ppm to 554 ppm.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
88
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Figure 2. Average COD concentration levels vs. treatment time and mass of Lemna Minor. Source: authors’ own elaboration.
Figure 2 shows the most signicant reduction in average COD concentration for a treatment time of 10
days and a mass of 100 g from 1747 to 554 ppm. With a treatment of 200 g of Lemna Minor, the COD
concentration decreases in 6 days from 1747 to 603 ppm and then increases in 10 days to 684 ppm. The
same behavior is obtained for treatment with 300 g of Lemna Minor, the COD concentration decreases
in 6 days from 1747 to 639 ppm, concluding that a better treatment of organic contamination expressed
as COD is obtained with a mass of 100 g of Lemna Minor.
Table 10. Distribution of TSS concentration averages vs. treatment time for a mass of 100 g of Lemna Minor.
Mass Lemna
Minor (g)
Time (days) Media SST (ppm)
100 0 124
100 3 82
100 6 60
100 10 48
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
89
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
In Table 10, the decrease of the average concentrations as a function of time of the total suspended
solids for a treatment with a mass of 100 g of Lemna Minor, the average concentration of TSS initially
was 124 ppm; when being in contact for 3 days, the average concentration of TSS decreases to 82 ppm;
for 6 days of contact, the concentration of TSS 60 ppm and in 10 days a concentration of TSS at 48
ppm is obtained, obtaining a considerable reduction.
Table 11. Distribution of TSS concentration averages vs. treatment time for 200 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (days) Media SST (ppm)
200 0 124
200 3 86
200 6 63
200 10 78
Source: authors’ own elaboration.
Table 11 show the decrease in the time-averaged concentrations of total suspended solids for treatment
with 200 g Lemna Minor. The average concentration of TSS initially was 124 ppm; when in contact for
3 days, the average concentration of TSS decreases to 86 ppm. On the other hand, for 6 days of contact,
the concentration of TSS is 63 ppm, and in 10 days, a concentration of TSS at 78 ppm is obtained,
obtaining a considerable reduction.
Table 12. Distribution of TSS concentration averages vs. treatment time for 300 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Time (days)
Media SST
(ppm)
300 0 124
300 3 75
300 6 51
300 10 99
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
90
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
In Table 12 the decrease of the average concentrations as a function of time of the total suspended solids
for a treatment with a mass of 300 g of Lemna Minor, the average concentration of TSS initially was
124 ppm; when being in contact for 3 days the average concentration of TSS decreases to 75 ppm; for 6
days of contact the concentration of TSS to 51 ppm and in 10 days a concentration of TSS to 99 ppm
is obtained, obtaining an increase.
Table 13. Distribution of TSS concentration averages vs. treatment time and mass of Lemna Minor.
MASA (gramos) TIEMPO (días) Media SST (ppm) Desviación estándar N
100 gramos
0 días 123,71 8,361 7
3 dias 82,29 6,157 7
6 dias 60 6,733 7
10 dias 48,43 4,685 7
Total 78,61 29,93 28
200 gramos
0 días 123,71 8,361 7
3 dias 85,86 6,283 7
6 dias 63 3,367 7
10 dias 77,71 4,923 7
Total 87,57 23,524 28
300 gramos
0 días 123,71 8,361 7
3 dias 74,71 7,544 7
6 dias 51,14 3,288 7
10 dias 98,57 2,507 7
Total 87,04 28,083 28
Total
0 días 123,71 7,932 21
3 dias 80,95 7,934 21
6 dias 58,05 6,845 21
10 dias 74,9 21,445 21
Total 84,4 27,295 84
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
91
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Figure 3. Average TSS concentration levels vs. treatment time and Lemna Minor mass. Source: authors’ own elaboration.
In Figure 3, it can be seen that the most signicant reduction in average TSS concentration is achieved
with a treatment time of 10 days and a mass of 100 g of 124 to 48 ppm. With a treatment of Lemna
Minor of 200 g, the concentration of TSS decreases in 6 days from 124 to 63 ppm. It then increases
in 10 days to 78 ppm; the same behavior is obtained for treatment with 300 g of Lemna Minor, the
concentration of TSS decreases in 6 days from 124 to 51 ppm and at 10 days increases to 99 ppm,
concluding that a better treatment of organic contamination expressed as TSS is obtained with a mass
of 100 g of Lemna Minor.
Table 14. Distribution of % Average removal of BOD concentration vs. treatment time for a mass of 100 g of Lemna Minor.
Masa Lemna
Minor (g)
Media DBO % Remotion
100 0-3 días 37
100 0-6 días 53
100 0-10 días 61
Source: authors’ own elaboration.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
92
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
12
Figure 4. Average % BOD removal concentration vs. treatment time for 100 g
mass of Lemna Minor. Source: authors’ own elaboration.
Table 14 and Figure 4 show the average levels of % removal of BOD BOD concentration vs.
treatment time for a treatment mass of 100 g of Lemna Minor. On the third day of treatment,
37%, on the sixth day, 53%, and the tenth day of treatment, 61% of average BOD removal was
obtained. It is concluded that for the treatment with a mass of 100 g of Lemna Minor, the
maximum removal of BOD is obtained at the tenth day of treatment.
Table 15. Distribution of % Average removal of BOD concentration vs. treatment time for
a mass of 200 g of Lemna Minor.
Mass Lemna
Minor (g)
Media DBO
% Remotion
200
0-3 días
43
200
0-6 días
58
200
0-10 días
53
Source: authors’ own elaboration.
Table 15 show the average levels of % removal of BOD concentration vs. treatment time for a
treatment mass of 200 g of Lemna Minor. On the third day of treatment, 43%, on the sixth day
of 58%, and the tenth day of treatment, 53% of average BOD removal was obtained. It is
concluded that for the treatment with a mass of 200 g of Lemna Minor, the maximum removal
of BOD is obtained on the sixth day of treatment.
Table 16. Distribution of average removal of BOD concentration vs. treatment time for a
300 g mass of Lemna Minor.
Mass Lemna
Minor (g)
Media DBO
% Remotion
300
0-3 días
48
300
0-6 días
60
0
10
20
30
40
50
60
70
0-3 dias 0-6 dias 0-10 dias
37 %
53 %
61 %
%
Days Treatment
% Average removal DBO (100 g)
Figure 4. Average % BOD removal concentration vs. treatment time for 100 g mass of Lemna Minor. Source: authors’ own
elaboration.
Table 14 and Figure 4 show the average levels of % removal of BOD BOD concentration vs. treatment
time for a treatment mass of 100 g of Lemna Minor. On the third day of treatment, 37%, on the sixth
day, 53%, and the tenth day of treatment, 61% of average BOD removal was obtained. It is concluded
that for the treatment with a mass of 100 g of Lemna Minor, the maximum removal of BOD is obtained
at the tenth day of treatment.
Table 15. Distribution of % Average removal of BOD concentration vs. treatment time for a mass of 200 g of Lemna Minor.
Mass Lemna
Minor (g)
Media DBO % Remotion
200 0-3 días 43
200 0-6 días 58
200 0-10 días 53
Source: authors’ own elaboration.
Table 15 show the average levels of % removal of BOD concentration vs. treatment time for a treatment
mass of 200 g of Lemna Minor. On the third day of treatment, 43%, on the sixth day of 58%, and
the tenth day of treatment, 53% of average BOD removal was obtained. It is concluded that for the
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
93
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
treatment with a mass of 200 g of Lemna Minor, the maximum removal of BOD is obtained on the
sixth day of treatment.
Table 16. Distribution of average removal of BOD concentration vs. treatment time for a 300 g mass of Lemna Minor.
Mass Lemna Minor
(g)
Media DBO % Remotion
300 0-3 días 48
300 0-6 días 60
300 0-10 días 35
Source: authors’ own elaboration.
13
300
0-10 días
35
Source: authors’ own elaboration.
Figure 15. Average % removal of BOD concentration vs. treatment time for 300 g mass
of Lemna Minor. Source: authors’ own elaboration.
In Table 16 and Figure 15, the average levels of % removal of concentration of the biochemical
demand of oxygen BOD versus the time of treatment for a treatment mass of 300 g of Lemna
Minor is observed, obtaining 48% on the third day of treatment, 60% on the sixth day and 35%
on the tenth day of treatment of average removal of BOD. It is concluded that for the treatment
with a mass of 300 g of Lemna Minor, the maximum removal of BOD is obtained on the sixth
day of treatment.
55.. DDiissccuussssiioonn
Sarango, nchez and Landívar (2016), in their experimental design and applied type, designed
2 bio-filters of 68 L capacity each. In contrast to the present research, both investigations were
carried out with Lemna Minor applied to industrial effluents, in which there is a percentage of
removal expressed as COD 72.57 %, BOD5 73.36 %, total solids 75.21 % and in the first six
days of treatment with different masses of 100g, 200g of Lemna Minor there is a high
percentage of decrease in the concentrations of the parameters mentioned expressed as organic
matter. In the treatment with 300 g of Lemna Minor, there is a decrease but not in the same
proportion. From the sixth day of treatment, there is a minimum reduction or increase
depending on the masses of Lemna Minor due to a stage of withering, which decreases its
capacity of assimilation.
But in both, it is concluded that there is a decrease of COD, BOD5, and TSS in the treatment
of industrial effluents with Lemna Minor.
66.. CCoonncclluussiioonn
0
10
20
30
40
50
60
0-3 dias 0-6 dias 0-10 dias
48 %
60 %
35 %
%
Treatement days
% media remotion DBO (300 g)
Figure 5. Average % removal of BOD concentration vs. treatment time for 300 g mass of Lemna Minor. Source: authors’ own
elaboration.
In Table 16 and Figure 5, the average levels of % removal of concentration of the biochemical demand
of oxygen BOD versus the time of treatment for a treatment mass of 300 g of Lemna Minor is observed,
obtaining 48% on the third day of treatment, 60% on the sixth day and 35% on the tenth day of
treatment of average removal of BOD. It is concluded that for the treatment with a mass of 300 g of
Lemna Minor, the maximum removal of BOD is obtained on the sixth day of treatment.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
94
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
5. DISCUSSION
Sarango, Sánchez and Landívar (2016), in their experimental design and applied type, designed 2 bio-
lters of 68 L capacity each. In contrast to the present research, both investigations were carried out
with Lemna Minor applied to industrial euents, in which there is a percentage of removal expressed as
COD 72.57 %, BOD5 73.36 %, total solids 75.21 % and in the rst six days of treatment with dierent
masses of 100g, 200g of Lemna Minor there is a high percentage of decrease in the concentrations of
the parameters mentioned expressed as organic matter. In the treatment with 300 g of Lemna Minor,
there is a decrease but not in the same proportion. From the sixth day of treatment, there is a minimum
reduction or increase depending on the masses of Lemna Minor due to a stage of withering, which
decreases its capacity of assimilation.
But in both, it is concluded that there is a decrease of COD, BOD5, and TSS in the treatment of
industrial euents with Lemna Minor.
6. CONCLUSION
Of the three treatments of 100g, 200g, and 300g of Lemna Minor, it is observed that the parameter of
BOD obtains a greater removal at 10 days of treatment with a mass of 100g Lemna Minor and 6 days
with 300g of Lemna Minor.
For the DQO in the treatments with 100g, 200g, and 300g of Lemna Minor, a greater removal is
obtained to the 10 days of treatment with a mass of 100g of Lemna Minor and the 6 days with 200g of
Lemna Minor.
For TSS in the treatments of 100g, 200g, and 300g of Lemna Minor, there is a greater removal at 10
days of treatment with a mass of 100g of Lemna Minor and at 6 days with 300g of Lemna Minor.
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
95
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
From the analysis of the parameters that measure organic contamination such as BOD, COD, and
TSS versus contact time we found that there is an inverse relationship, as contact time increases these
parameters decrease, but in the tests carried out with 200 and 300g it increases from the 6th day, this
occurs due to the increase of Lemna Minor that dies during the treatment.
REFERENCES
Coronel, E. (2016). Eciencia del Jacinto de agua (Eichhornia Crassipes) y Lenteja de agua (Lemna Minor) en
el tratamiento de las aguas residuales de la Universidad Nacional Toribio. Facultad de Ingeniería Civil y
ambiental. Universidad Nacional “Toribio Rodríguez de Mendoza de Amazonas”. http://
repositorio.untrm.edu.pe/bitstream/handle/UNTRM/657/EFICIENCIA%20DEL%20
JACINTO%20DE%20AGUA.pdf ?sequence=1&isAllowed=y
Dirección General de Salud Ambiental e Inocuidad Alimentaria (DIGESA). (s.f.). Ministerio
de Salud. Perú. http://www.digesa.minsa.gob.pe/
Esenarro, D., Rodriguez, C., Aquije, C., Obregon, N., Anicama, L., & Arguedas, C.
(2020). Cable Car with Water Collection for Aorestation of the Solar Hill in Chorrillos,
Perú. Test Engineering & Management, 83, 9236 – 9242.
http://www.testmagzine.biz/index.
php/testmagzine/article/view/5279
Hoyos, A., Ramirez, A., Fernandez, V., & Sanchez, N. E. (2016). Lenteja de agua (Lemna minor)
para el tratamiento de las aguas residuales que provienen del lavado de la bra de que (Furcraea
bedinghausii). Ingenieria y Competividad, 18(2), 25-34. https://doi.org/10.25100/iyc.v18i2.2151
Jojoa, G., Rodríguez, H., & Cardona, S. (2015). Tratamiento de aguas residuales textiles a partir
de métodos biológicos. Revista CINTEX, 20(1), 11-34. https://www.academia.edu/38191080/
Tratamiento_de_aguas_residuales_textiles_a_partir_de_m%C3%A9todos_biol%C3%B3gicos
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
96
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97
Li, H., Mo, F., Li, Y., Wang, M., Li, Z., Hu, H., Deng, W., & Zhang, R. (2020). Eects of silver
(I) toxicity on microstructure, biochemical activities, and genic material of Lemna minor L. with
special reference to application of bioindicator. Environmental Science & Pollution Research, 27(18),
22735-22748. https://doi.org/10.1007/s11356-020-08844-8
Rodriguez, C., Esenarro, D., Ccorimanya, P., Flores, F., Aylas, C., & Lagos, J. (2020).
Proposal for a sustainable infrastructure design (ecolodge) in the Quichas Town, Perú. Test
Engineering and Management, 83, 9250-9256. http://www.testmagzine.biz/index.php/testmagzine/
article/view/5281
Sarango, J. A., Sánchez, S., & Landívar, J. (2016). Educación ambiental. ¿Por qué la Historia?.
Revista Universidad y Sociedad, 8(3), 184-187. http://scielo.sld.cu/scielo.php?script=sci_
abstract&pid=S2218-36202016000300025&lng=es&nrm=iso
Sun, Y., Gao, P., Ding, N., Zou, X., Chen, Y., Li, T., Cuiting, W., Xu, X., Chen, T., Ruan, H.
(2020). Feasible Green Strategy for the Quantitative Bioaccumulation of Heavy Metals by Lemna
minor: Application of the Self-Thinning Law. Bulletin of Environmental Contamination & Toxicology,
104(2), 282-287. https://doi.org/10.1007/s00128-019-02772-1
Walsh, É., Paolacci, S., Burnell, G., Jansen, M. A. K. (2020). The importance of the calcium-to-
magnesium ratio for phytoremediation of dairy industry wastewater using the aquatic plant Lemna
minor L. International Journal of Phytoremediation, 22(7), 694-702. https://doi.org/10.1080/152265
14.2019.1707478
3C Tecnología. Glosas de innovación aplicadas a la pyme. ISSN: 2254 – 4143 Ed. 35 Vol. 9 N.º 3 Septiembre - Diciembre
97
https://doi.org/10.17993/3ctecno/2020.v9n3e35.77-97